Semi Prep HPLC Column, Diamond Hydride, 4 um, 100 A. 10 mm ID x 250 mm Length. Cogent TYPE-C Brand. 1 EA.
Additional Info:
The 70000‑SP250 Diamond Hydride Semi‑Preparative HPLC Column is designed for high‑resolution, application‑critical purification workflows where shorter semi‑prep columns do not provide sufficient separation. This extended‑length format delivers maximum resolving power while allowing collection of milligram‑level quantities of target analytes.
Semi‑Preparative Use Case: Why 10 mm ID × 250 mm
The 10 mm internal diameter defines this column as semi‑preparative, ideal for laboratories scaling HILIC methods beyond analytical formats without moving to full preparative systems.
The 250 mm column length provides:
Maximum theoretical plate count within the semi‑prep class
Superior separation of closely eluting or structurally similar compounds
Higher peak capacity for very complex polar mixtures
Increased confidence during fraction collection and purification
This configuration is typically selected when resolution is the dominant requirement, and shorter 50 mm, 100 mm, or even 150 mm semi‑prep columns cannot adequately resolve target compounds.
Diamond Hydride™ TYPE‑C Stationary Phase Advantages
Manufactured with Cogent TYPE‑C™ silica hydride technology, the Diamond Hydride stationary phase provides proven performance advantages for demanding semi‑prep HILIC separations:
Highly stable and reproducible retention for polar analytes
Faster equilibration compared to conventional bonded‑silica HILIC phases
Robust column lifetime, even under high‑organic mobile‑phase conditions
This chemistry is especially effective for purification of:
Peptides and peptide‑like compounds
Metabolites and highly polar small molecules
Analytes containing amine and basic functional groups
Other compounds best isolated using HILIC‑based separations
Purpose‑Built for Maximum‑Resolution Scale‑Up
The 70000‑SP250 enables predictable scale‑up from analytical and shorter semi‑prep methods while preserving chromatographic behavior. It is frequently used in advanced research, method‑critical purification, and regulated workflows where separation quality cannot be compromised.
System Requirements
Typical operating flow rates: ~3–8 mL/min
Requires a semi‑preparative HPLC system capable of:
Sustained higher‑flow delivery
Larger injection volumes
Appropriate detector configuration and fraction collection
Standard analytical HPLC systems are not suitable for 10 mm ID semi‑preparative columns.
Flow rates may be optimized further based on mobile‑phase viscosity, pressure limits, and purification objectives.
Made‑to‑Order Columns & Pricing Information
These columns represent a significant investment and are often application‑specific. To ensure customers receive the correct column for their workflow:
Pricing and technical support is provided through our Customer Service Department via formal quotation. Use our online form to start the process or call customer service.
Columns are produced on order, based on customer requirements
Columns cannot be returned or orders canceled once placed
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